Spin Column 150 Kda - PROMOLET.NETLIFY.APP

Zeba Desalting Products | Thermo Fisher Scientific - MX.
Desalting and Buffer Exchange with Corning’s Spin-X UF.
Vivaspin protein concentrator spin columns | Cytiva.
Amintra (R) Desalting Spin Columns (ADS0050): Novus Biologicals.
Bio-Spin® 6 and Micro Bio-Spin™ 6 Columns | Bio-Rad Laboratories.
PDF Biotinylation of Proteins for Immobilization Onto Streptavidin Biosensors.
Sodium Azide Removal Protocol - Creative Biolabs.
Ezh2 Antibody - Cell Signaling Technology.
Biotin-XX Microscale Protein Labeling Kit.
Characterization of Microfibrillar-associated Protein 4 (MFAP4) as a.
PDF Ultra-15 How to Use Amicon® Ultra-15 Centrifugal Filter Devices.
PDF AnaTag™ HiLyte Fluor™ 555 Microscale Protein Labeling Kit - Anaspec.
Ni-NTA Spin Kit Handbook - Qiagen.
Strep-tag®: leading affinity tag in recombinant protein production.

Zeba Desalting Products | Thermo Fisher Scientific - MX.

Zeba™ Spin Desalting Columns, (40K MWCO or 7K, 0.5 mL: this size allows max 130ul protein sample for loading) works beautifully if you just follow the product manual. It is very fast with high. Mini Spin Column: #1910-050/250, no lid. #1920-050/250, w/ lid. Two types to choose from: with attached lid and without lid. Designed with extra considerations for better performance and convenience. Improved o-ring configuration completely eliminates buffer carryover, cleaner DNA for sequencing, digestion and transfection. GppNHp was removed by spin column. Ready for use in KRAS-RAF binding studies or inhibitor assays. Assay Conditions: KRAS (G12C) GppNHp-loaded was tested for binding to the RBD of Raf1.... -150 kDa - 100 kDa - 75 kDa - 50 kDa - 37 kDa - 25 kDa - 20 kDa - 15 kDa - 250 kDa - 10 kDa. Email: 6042 Cornerstone Court West, Suite B.

Desalting and Buffer Exchange with Corning’s Spin-X UF.

Chromatography Columns. Pre-Packed Columns; Automated Columns; Manual Columns; Chromatography Systems. Multi-Use Batch Chromatography Systems;... 300 kDa Performance Characteristics. Time to concentrate up to 30x [min.] at 20°C Concentrate recovery % Start volume: 500 μl: 500 μl: 3,000 MWCO PES: 30: 96 %: 5,000 MWCO PES: 15. Systemic and tumor PK of trastuzumab ( ;150 kDa), FcRn-nonbinding trastuzumab (;150 kDa), F(ab) 2 fragment of trastuzumab ( ;100 kDa),... Briefly, 4 mg of trastuzumab was first purified using the Zeba Spin Desalting column (Thermo Fisher Scientific, Waltham, MA) by centrifugation at 1000g for 2 minutes (three times to tally). Then, purified.

Vivaspin protein concentrator spin columns | Cytiva.

The spin column matrix binds up to 4 nmol recombinant Strep-tag fusion protein (corresponding to 150 µg of a 37 kDa protein (GAPDH-Strep-tag)) Buffer W (washing buffer): 100 mM Tris∙Cl, 150 mM NaCl, 1 mM EDTA, pH 8 Buffer BE (elution buffer): 100 mM Tris∙Cl, 150 mM NaCl, 1 mM EDTA, 2 mM D-biotin, pH 8.

Amintra (R) Desalting Spin Columns (ADS0050): Novus Biologicals.

4 Ni-NTA Spin Kit Handbook 01/2008 Kit Contents Ni-NTA Spin Ni-NTA Columns Spin Kit Catalog No. 31014 31314 Ni-NTA Spin Columns 50 50 2 ml Collection Microtubes 50 50 Guanidine HCl 40 g Urea 100 g 1 M Imidazole, pH 7.0 50 ml 5x Phosphate Buffer Stock Solution (0.5 M NaH 2 PO 4; 50 mM Tris·Cl, pH 8.0) 100 ml Control Vector DNA 1 µg Storage. Elution was performed by incubation with 150 µL of a solution consisting of H 2O/IPA (1:1) and formic acid (1% v/v) for 5 minutes (shaking at room temperature, 750 rpm). The eluted protein was subsequently desalted and solvent exchanged to 150 mM ammonium acetate by using size exclusion spin columns (7 kDa molecular cut-oﬀ), operated.

Bio-Spin® 6 and Micro Bio-Spin™ 6 Columns | Bio-Rad Laboratories.

With molecular weights between 12 and 150 kDa, and contains everything needed to perform three labeling reactions and to separate the resulting conjugates from excess reactive biotin. Convenient spin columns are used to purify the labeled protein with yields between 60 and 90%, depending primarily on the molecular weight of the starting material. The exoEasy Maxi Kit uses membrane affinity spin columns to efficiently isolate exosomes and other extracellular vesicles from serum, plasma, cell culture supernatant and other biological fluids. The maxi column format allows the use of large sample volumes, including up to 4 ml plasma or serum or up to 32 ml cell culture supernatant. He compared the 10KD, 30Kd, 50KD filters and found that filters with larger nominal molecular weight cut-offs of 30 and 50 k, as opposed to the originally described 10 k ones, have advantages in peptide yield and sample preparation time. In their recently papers, they preferred to use the 30KD filters. As Cristopher correctly pointed, there is.

PDF Biotinylation of Proteins for Immobilization Onto Streptavidin Biosensors.

Column. Strep-Tactin®XT Spin Columns cannot be re-used. Protocol 1. Centrifuge cleared lysates (13.000 rpm, 5 min, 4°C, microfuge). Insoluble aggregates which may have formed after storage may clog the column and thus have to be removed. 2. Equilibrate the Strep-Tactin®XT Spin Column with 2x 500 µl Buffer W. Amicon ® Ultra centrifugal filter devices provide fast ultrafiltration with high concentration factors from dilute and complex sample matrices. The vertical design and membrane surface area provide fast sample processing, high sample recovery, and the capability for up to 80-fold concentration factors. Features.

Sodium Azide Removal Protocol - Creative Biolabs.

The size-exclusion column was calibrated with Rubisco (550 kDa), GAPDH (150 kDa), spinach PRK (80 kDa) and peroxidase (50 kDa). SDS-PAGE and dot blot experiments Sodium dodecyl sulphate polyacrylamide gel electrophoresis (12% acrylamide) was carried out in a Bio-Rad Mini Protean system. Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol... Blue Prestained Protein Marker, Broad Range (11-250 kDa):.... Remove the spin column from the collection tube and discard the liquid. Replace spin column in the collection tube..

Ezh2 Antibody - Cell Signaling Technology.

The spin columns included in the kits are used for purifying the labeled protein from excess dye reagents with yields of... For example, to label 100 μg of 150 kDa IgG at an MR of 10, you will need: (100 μg/150 kDa) × 10 = 3.3 μL of 2 mM reactive dye to add to sample. B. Protocol: TALON Spin Column Purification 1. Hold the TALON Spin Column upright and flick it until all resin falls to the bottom of the column. Then, snap off the breakaway seal. NOTE: Save end-cap for later use. 2. Place column in the 2 ml collection tube. 3. Remove the clear top-cap and centrifuge column at 700 x g for 2 min to remove the. Size-exclusion chromatography (on a calibrated Fractogel EMD BioSEC column) revealed a mass of 80 ± 5 kDa for the purified recombinant enzyme. Furthermore, the enzyme activity appeared to be independent of Mg 2+ , but was significantly reduced by the addition of 1.5 m m CaCl 2 or CoCl 2 , and almost completely abolished in the presence of 1.5.

Biotin-XX Microscale Protein Labeling Kit.

Vivaspin ® centrifugal concentrators are disposable ultrafiltration devices for the concentration of biological samples. Concentrate recoveries of > 90% can be reached in less than 30 min.

Characterization of Microfibrillar-associated Protein 4 (MFAP4) as a.

They effectively clean and remove salts from protein samples in just 10 minutes. Bio-Spin 6 and Micro Bio-Spin 6 columns: Provide fast salt and contaminant removal in an easy-to-use spin-column format. Remove compounds <6 kD by size exclusion chromatography. Accommodate up to 100 µl of sample. The eluate was concentrated by ultrafiltration (Amicon, MWCO 10 kDa), and free biotin was removed by gel filtration (Zeba spin columns, MWCO 7 kDa, ThermoFisher). The concentration of EL2BH was estimated on a Shimadzu biospec-nano, using a molar mass of 19,531 Da and a molar extinction coefficient of 39,545/M/cm. Immunoprecipitation. After the removal of the Pup ligase Cglu PafA, the reaction buffer was exchanged to a phosphate buffer containing 50 mM NaHPO 4, 150 mM NaCl and 0.5 mM EDTA supplemented with Inhibitor Cocktail (Roche) using an Amicon spin column with 30 kDa molecular weight cutoff in the case of Mtb Pup ~ Mtb FabD or Mtb Pup ~ Mtb PanB and 3 kDa molecular.

PDF Ultra-15 How to Use Amicon® Ultra-15 Centrifugal Filter Devices.

Column packing and equilibration ( 2 h), 20-30 min puri ca-... (150 kDa), were not removed as eﬃ-ciently due to the smaller pore diameter (data not shown).... a 146 kDa pharmaceutical antibody, was selected as an example of removal of unbound, full length antibodies from liposome preparations.1,12,22,23 For. Spin column technology is a simple and quick approach to extracting nucleic acids from small biological samples.... (10 mM Tris-base, pH 7.4, 150 mM NaCl, 0.1% Tween-20, and deionized water). Membranes were then washed in TBST and immunoblotted with anti-α-actin A4700 (Sigma-Aldrich, Castle Hill, NSW, Australia) in 5% milk rocking overnight. Place the RNeasy spin column in a new 2 ml collection tube, and discard the old collection tube with the flow-through.... ‒ 150 mM NaCl ‒ 1.0% NP-40 (possible to substitute with 0.1% Triton X-100)... For proteins larger than 80 kDa, we recommend that SDS is included at a final concentration of 0.1%. Blocking buffer 3-5% milk or BSA.

PDF AnaTag™ HiLyte Fluor™ 555 Microscale Protein Labeling Kit - Anaspec.

With the most comprehensive range of centrifugal concentrators, comprising 12 device types, Sartorius offers the optimal solution to any ultrafiltration or buffer exchange application. The Vivaspin ® and Vivacon ® families provide a wide choice of sample capacities, membrane materials and MWCOs. Large active membrane areas and integrated dead. After the column has settled, rinse the 50-ml conical tube with another 5 ml of wash buffer. 18 Elute with 6 ml elution buffer (50 mM Tris (pH 8), 150 mM NaCl, and 0.5 M imidazole) into a 15-ml tube.

Ni-NTA Spin Kit Handbook - Qiagen.

1 – 14. Maximum centrifugal force. 7,500 x g (for ultrafiltration) Centrifuge. Fits rotors that work with standard 15 mL conical tubes and are capable of 3,000 to 14,000 x g. Sanitization. Non-sterile. May be sanitized by filtering 70% ethanol through the device before use. Macrosep® Centrifugal Filters. Molecular weight of your protein in kDa, MR is the dye:protein molar ratio from Table 2, and 2 μg/μL is the concentration of the reactive dye stock solution (2 mM). For example, to label 100 μg of 150 kDa IgG at an MR of 10, you will need: (100 μg/150 kDa) × 10 = 3.3 μL of 2 mM reactive dye to add to sample 2 μg/μL.

Strep-tag®: leading affinity tag in recombinant protein production.

Microfiltration Durapore® PVDF cut discs are available 0.1 µm, 0.22 µm, and 0.45 µm pore sizes in both 63.5 mm and 75 mm diameters. Features & Benefits: •Disc diameters include: 25, 44.5, 47, 63.5, 76, 90 and 150 mm. •Both Ultracel®, Durapore®, and Biomax® membranes are available in a wide range of pore sizes to meet your separation. Zeba Spin Desalting Columns, 0.5 mL (7K MWCO) (Cat. No. 89882), GE SpinTrap G-25 and Bio-Rad Micro BioSpin 6 spin columns were used to desalt 40, 80 and 120 μL samples of a HeLa lysate at a concentration of 0.2 and 1 mg/mL. Desalting was performed according to the manufacturers' recommended protocols. Protein recovery was analyzed by SDS-PAGE.